Peer-reviewed Publications

 

 

 
Abdeladim L, Matho KS, Clavreul S, Mahou P, Sintes JM, Solinas X, Arganda-Carreras I, Turney SG, Lichtman JW, Bemelmans AP, Loulier K, Supatto W, Livet J, Beaurepaire E: Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy.
Nature communications, 10, 1662 (2019).
Pubmed | Nature Publishing Group

CNRS news (in french)
INSERM news
Ecole Polytechnique Video
TheScientist image of the day
 


 

 

 
Morizet J, Ducourthial G, Supatto W, Boutillon A, Legouis R, Schanne-Klein MC, Stringari C, Beaurepaire E: High-speed polarization-resolved third-harmonic imaging.
Optica, 6(3), 385-388 (2019).
Pubmed | OSA publishing

Research Highlight in Nature Photonics
 
 

 

 
Ferreira RR, Pakula G, Klaeyle L, Fukui H, Vilfan A, Supatto W, Vermot J: Chiral cilia orientation in the left-right organizer.
Cell reports, 25(8), 2008-2016 (2018).
Pubmed | Cell press
 


 

 

 
Guesmi K, Abdeladim L, Tozer S, Mahou P, Kumamoto T, Jurkus K, Rigaud P, Loulier K, Dray N, Georges P, Hanna M, Livet J, Supatto W, Beaurepaire E, Druon F: Dual-color deep-tissue three-photon microscopy with a multiband infrared laser.
Light: Science & Applications, 7(1), 12 (2018).
Pubmed | Nature Publishing Group
 


 

 

 
Mahou P, Malkinson G, Chaudan E, Gacoin T, Beaurepaire E, and Supatto W: Metrology of multiphoton microscopes using second harmonic generation nanoprobes.
Small, 13(42), 1701442 (2017).
Pubmed | Wiley Online Library
 


 

 

 
Ferreira RR, Vilfan A, Jülicher F, Supatto W*, Vermot J*: Physical limits of flow sensing in the left-right organizer.
* equal contribution
eLife, 6:e25078 (2017).
Pubmed | ELife Sciences
 
 

 

 
Cadroas P, Abdeladim L, Kotov L, Likhachev M, Lipatov D, Gaponov D, Hideur A, Tang M, Livet J, Supatto W, Beaurepaire E and Février S: All-fiber femtosecond laser providing 9 nJ, 50 MHz pulses at 1650 nm for three-photon microscopy.
Journal of Optics, 19(6), 065506 (2017).
Pubmed | IOPscience
 


 

 

 
Stringari C, Abdeladim L, Malkinson G, Mahou P, Solinas X, Lamarre I, Brizion S, Galey JB, Supatto W, Legouis R, Pena AM, Beaurepaire E: Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing.
Scientific Reports, 7, 3792 (2017).
PubmedNature Publishing Group
 


 

 

 
Vuillemin N, Mahou P, Debarre D, Gacoin T, Tharaux PL, Schanne-Klein MC, Supatto W, and Beaurepaire E: Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation.
Scientific Reports, 6, 29863 (2016).
PubmedNature Publishing Group
 


 

 

 
Wolf S, Supatto W, Debregeas G, Mahou P, Kruglik SG, Sintes JM, Beaurepaire E, and Candelier R: Whole-brain functional imaging with two-photon light-sheet microscopy.
Nature Methods, 12(5), 379-380 (2015).
PubmedNature Publishing Group 
 


 

 

 
Dray N, Bedu S, Vuillemin N, Alunni A, Coolen M, Krecsmarik M, Supatto W, Beaurepaire E, and Bally-Cuif L: Large-scale live imaging of adult neural stem cells in their endogenous niche.
Development, 142(20),3592-3600 (2015).
Pubmeddev.biologists.org 
 
 

 

 
Mahou P, Vermot J, Beaurepaire E, and Supatto W: Multicolor two-photon light-sheet microscopy.
Nature Methods, 11(6), 600-601 (2014).
PubmedNature Publishing Group 

Highlighted in Ten years of Methods, Nature Methods, 11, 1000-1001 (2014) | see Methagora post
CNRS news (in french)


 

 

 
Pantazis P and Supatto W: Advances in whole-embryo imaging: a quantitative transition is underway.
Nature Reviews Molecular Cell Biology, 15(5), 327-339 (2014).
PubmedNature Publishing Group
 


 

 

 
Debarre D, Olivier N, Supatto W, and Beaurepaire E: Mitigating phototoxicity during multiphoton microscopy of live Drosophila embryos in the 1.0-1.2 μm wavelength range.
PLOS One, 9(8), e104250 (2014).
Pubmed | plosone.org

 


 

 

 
Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS,Ieng S, Fouquet S, Dupin E, Benosman R, Chedotal A, Beaurepaire E, Morin X, and Livet J: Multiplex cell lineage tracking with combinatorial labels.
Neuron, 81(3), 505-520 (2014).
Pubmed| Cell Press
 


 

 

 
Mahou P, Zimmerley M, Loulier K, Matho KS, Labroille G, Morin X, Supatto W, Livet J, Debarre D, and Beaurepaire E: Multicolor two-photon tissue imaging by wavelength mixing.
Nature Methods, 9(8), 815-818 (2012).
PubmedNature Publishing Group | 
 


 

 

 
Supatto W, Truong TV, Debarre D, and Beaurepaire E: Advances in multiphoton microscopy for imaging embryos.
Current Opinion in Genetics and Development, 21(5), 538-548 (2011).
Pubmed | ScienceDirect
 


 

 

 
Truong TV*, Supatto W*, Koos DS, Choi JM, and Fraser SE: Deep and fast live imaging with two-photon scanned light-sheet microscopy.
* equal contribution
Nature Methods, 8(9), 757-760 (2011).
Pubmed | Nature Publishing Group

Nature Collections
Highlighted in Ten years of Methods, Nature Methods, 11, 1000-1001 (2014) | see Methagora post
Cell Observatory Publication of the Week
 

 

 

 

 
Truong TV, and Supatto W: Toward high-content/high-throughput imaging and analysis of embryonic morphogenesis.
Genesis, 49(7), 555-569 (2011).
Pubmed | Wileypdf
 


 

 

 
Supatto W, and Vermot J: From cilia hydrodynamics to zebrafish embryonic development.
Current Topics in Developmental Biology, 95, 33-66 (2011).
Pubmed | ScienceDirectpdf
 


 

 

 
McMahon A, Reeves G, Supatto W, and Stathopoulos A: Mesoderm migration in Drosophila is a multi-step process requiring FGF signaling and integrin activity.
Development, 137(13), 2167-2175 (2010).
Pubmed | dev.biologists.org | Free on PMC

 

 

 

 

 
Supatto W, McMahon A, Fraser SE, and Stathopoulos A: Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysis.
Nature Protocols, 4(10), 1397-1412 (2009).
Pubmed | Nature Publishing Group | Free on PMC

 

 

 

 

 
McMahon A*, Supatto W*, Fraser SE, and Stathopoulos A: Dynamic analyses of Drosophila gastrulation provide insights into collective cell migration.
* equal contribution
Science, 322(5907), 1546-1550 (2008).
Pubmed | Scopus | Science website | Free on PMC

"Complex dance of embryo cells filmed in new detail": New Scientist (2008) link
Drosophila Image Award (2009)
American Society of Cell Biology - Celldance Video Winner (2009)

 


 

 

 
Supatto W, Fraser SE, and Vermot J: An all-optical approach for probing microscopic flows in living embryos.
Biophysical Journal, 95(4), L29-L31, (2008).
Pubmed | Scopus | Cell Press | Free on PMC

"Going with the flow": News in Analytical Chemistry (2008) full text
"4D-particle tracking": Show case of Imaris (Bitplane AG, Switzerland)
 


 

 

 
Desprat N, Supatto W, Pouille PA, Beaurepaire E, and Farge E: Tissue deformation modulates Twist expression to determine anterior midgut differentiation in Drosophila embryos.
Developmental Cell, 15(3), 470-477 (2008).
Pubmed | Scopus | Cell Press

"Giving a Twist to Twist": Editor's Choice in Science, 322(5902), 653 (2008). full text
"Cell biology: Stretching the imagination": News Feature in Nature, 456(7223), 696-699 (2008). full text
 


 

 

 
Debarre D, Supatto W, Pena AM, Fabre A, Tordjmann T, Combettes L, Schanne-Klein MC, and Beaurepaire E: Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy.
Nature Methods, 3(1), 47-53 (2006).
Pubmed | Scopus | Nature Publishing Group

Selected as cover page article.
"Great Balls of Fat": News Focus in Science, 311(5765), 1232-1234 (2006) full text
 


 

 

 
Debarre D, Pena AM, Supatto W, Boulesteix T, Strupler M, Sauviat MP, Martin JL, Schanne-Klein MC, and Beaurepaire E: Second- and third-harmonic generation microscopies for the structural imaging of intact tissues.
M/S - Medecine Sciences, 22(10), 845-852 (2006).
PubmedFree full text
 


 

 

 
Supatto W, Debarre D, Moulia B, Brouzes E, Martin JL, Farge E, and Beaurepaire E: in vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses.
PNAS, 102(4), 1047-1052 (2005).
Pubmed | Scopus | PNAS websiteFree on PMC 

"Making the kindest cut": Research Higlights in Nature Methods, 2, 155 (2005). full text
"Lasers used to see if function follows form", Biophotonics International 12(3), 51-2 (2005).
 


 

 

 
Debarre D, Supatto W, and Beaurepaire E: Structure sensitivity in third-harmonic generation microscopy.
Optics Letters, 30(16), 2134-2136 (2005).
Pubmed | Scopus | Optical Society of America
 


 

 

 
Supatto W, Debarre D, Farge E, and Beaurepaire E: Femtosecond pulse-induced microprocessing of live Drosophila embryos.
Medical Laser Application, 20(3), 207-216 (2005).
Scopus | ScienceDirect
 


 

 

  
Debarre D, Supatto W, Farge E, Moulia B, Schanne-Klein MC, and Beaurepaire E: Velocimetric third harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos.
Optics Letters, 29(24), 2881-2883 (2004).
Pubmed | Scopus | Optical Society of America
 


 

 

 
Brouzes E, Supatto W, and Farge E: Is mechano-sensitive expression of twist involved in mesoderm formation?
Biology of the Cell, 96(7), 471-477 (2004).
Pubmed | Scopus | ScienceDirect
           

 


 



 

Other
Publications
[in french]

 

 

 
Supatto W: Microscopie multiphoton illuminée par nappe : imagerie de fluorescence rapide et en profondeur dans les tissus biologiques
Photoniques, 62, 33-37 (2012).
Photoniques.compdf
 


 

 

 
Supatto W: Quantitative multiphoton imaging and femtosecond pulse-induced ablation to study mechano-sensitive gene expression during early Drosophila embryo development (Paris Diderot University / Institut Curie / Ecole Polytechnique)
PhD Thesis (2005). 
Full text in French












   




 



 
Patents
  WO/2013/113861
Ecole Polytechnique & CNRS / Mahou P, Beaurepaire E, Debarre D, Supatto W, and Martin JL: Multicolour excitation module for a multiphoton imaging system, and associated method and system
International patent application PCT/EP2013/052002
Publication date: August 8, 2013


 

  WO/2011/059826
California Institute of Technology / Truong TV, Supatto W, Koos DS, Choi JM and Fraser SE: Multiple-photon excitation light-sheet illumination microscope
International patent application PCR/US2010/054760
Invention Disclosure & Priority date: Oct 29, 2009 / Publication date: May 19, 2011


 

  WO/2011/059833
California Institute of Technology / Truong TV, Choi JM, Koos DS, Supatto W, and Fraser SE: Dual-mode raster point scanning / light-sheet illumination microscope
International patent application PCR/US2010/054809
Invention Disclosure & Priority date: Oct 29, 2009 / Publication date: May 19, 2011
 




  




 




 

Conference & Seminar Presentations

 

  • Topical Problems of Biophotonics 2015 - Russian Academy of Science International Symposium (invited speaker, 2015, Nizhny Novgorod, Russia).
  • Optique Bretagne - Symposium of the French Society of Optics (invited speaker, 2015, Rennes, France).
  • Imperial College - FILM Microscopy Day  (invited speaker, 2015, London, UK).
  • INRA - Symposium Microscopie Photonique (invited speaker, 2015, Jouy en Josas, France).
  • Paris University - 13e Journee Scientifique Imagerie Cellulaire du RIC de l'UPMC (invited speaker, 2015, Paris, France)
  • Bitplane Advanced Imaging Centers - Annual Meeting (invited partner, 2015, Zurich, Switzerland).
  • SPIE International Symposium - Photonics West 2015 (oral presentation, 2015, San Francisco, California, USA).
  • Harvard & MIT Broad Institute - VIZBI2015: Visualizing Biological Data (invited speaker & session chair, 2015, Cambridge, MA, USA).
  • IMB & Olympus workshop on Advanced Live-Cell Imaging (invited speaker, 2014, Singapore).
  • MIFOBIO 2014 - School on Functional Imaging in Biology (invited speaker, 2014, Seignosse, France).
  • Advances in photonic tools & technique for the life sciences - Cambridge University (invited speaker, 2014, Cambridge, UK).
  • Integrating modalities and scales in life science imaging - The Ohio State University (invited speaker, 2014, Columbus, OH, USA).
  • Nikon symposium on Microscopy Across Scales - Max Planck Institute of Molecular Cell Biology (invited speaker, 2014, Dresden, Germany.
  • International Conference on Physics & Biological Systems (organizer & poster presentation, 2014, Gif-sur-Yvette, France).
  • Bitplane Advanced Imaging Centers - Annual Meeting (invited partner, 2013, New Orleans, LA, USA).
  • ETH Zurich - Biosystems Science & Engineering Dept Seminar (invited seminar, 2013, Basel, Switzerland).
  • 1st TEFOR Symposium (invited speaker, 2013, Paris, France).
  • SFmu 2013: Colloque de la Société Française des Microscopies (oral presentation,2013, Nantes, France).
  • Université Paris Descartes - Neurophotonics Seminars (invited speaker, 2013, Paris, France).
  • IPCMS - Université de Starsbourg (invited seminar, 2013, Strasbourg, France).
  • Institut des Neurosciences de Montpellier (invited seminar, 2013, Montpellier, France).
  • 11e Colloque de la Société Française des Neurosciences (invited speaker, 2013, Lyon, France).
  • QuanTissue Symposium on Mechanics of Tissue - ESF RNP (invited speaker, 2012, Ljubljana, Slovenia).
  • Bitplane Advanced Imaging Centers - Annual Meeting (invited partner, 2012, Caltech, California, USA).
  • CNRS school on Multiphoton Microscopy in Life Sciences (organizer & speaker, 2012, Palaiseau, France).
  • BioVis 2011 - IEEE Symposium on Biological Data Visualization (invited speaker, 2011, Providence, RI, USA).
  • 3rd Light Sheet-based Fluorescence Microscopy Workshop (invited speaker, 2011, Toulouse, France).
  • SPIE/OSA - European Conference on Biomedical Optics (invited speaker, 2011, Munich, Germany).
  • Bitplane Advanced Imaging Centers - Annual Meeting (invited partner, 2011, EMBL, Heidelberg, Germany).
  • Orsay University - Soft Matter Department (invited seminar, 2011, Orsay, France).
  • Ecole Polytechnique (invited seminar, 2011, Palaiseau, France).
  • Harvard & MIT - VIZBI2011: Visualizing Biological Data (invited speaker, 2011, Cambridge, MA, USA).
  • University of Tokyo - Global Center of Excellence Program / International Symposium (invited speaker, 2010, Tokyo, Japan).
  • Ecole Normale Superieure (invited seminar, 2010, Paris, France).
  • Collège de France - Inaugural Symposium ITMO BCDE (invited speaker, 2010, Paris, France).
  • 2nd Light Sheet-based Fluorescence Microscopy Workshop (invited speaker, 2010, Dubin, Ireland).
  • Japanese Society of Developmental Biologists - 43rd Annual Meeting (invited speaker, 2010, Kyoto, Japan).
  • NIBB - National Institute for Basic Biology (invited seminar, 2010, Okazaki City, Japan).
  • NIG - National Institute of Genetics (invited seminar, 2010, Mishima, Japan).
  • 51st Annual Drosophila Research Conference (oral presentation, 2010, Washington DC, USA).
  • Workshop on early mammalian embryogenesis (oral presentation, 2010, Warsaw, Poland).
  • IGBMC (invited seminar, 2010, Strasbourg, France).
  • IST Austria - "Biology meets physics" symposium (invited speaker, 2009, Vienna, Austria).
  • The Rockefeller University - Microscopy Workshop (invited speaker, 2009, New York, USA).
  • Bitplane Web Seminar (invited seminar, 2009, WWW).
  • Morphogenesis & Regenerative Medicine Symposium (invited speaker, 2009, Charlottesville, Virginia, USA).
  • Tissue Dynamics & Growth - European Science Foundation workshop (invited speaker, 2009, Barcelona, Spain).
  • Caltech Biological Network Modeling Center seminar series (invited seminar, 2009, Pasadena, California, USA).
  • Bitplane Advanced Imaging Centers - Annual Meeting (invited partner, 2009, San Francisco, California, USA).
  • UC Berkeley - Advanced Imaging Methods Workshop (invited speaker, 2009, Berkeley, California, USA).
  • SPIE International Symposium - Photonics West (invited speaker, 2009, San Jose, California, USA).
  • Caltech Computational Morphodynamics Meeting (invited seminar, 2009, Pasadena, California, USA)
  • Institut Curie (invited seminar, 2008, Paris, France).
  • Asilomar IEEE Conference - Advances in Bioimaging and Analysis (invited speaker, 2008, Pacific Groove, California, USA).
  • GEM4 Summer School on Cell and Molecular Mechanics in Biomedicine (invited speaker, 2008, Caltech, Pasadena, California, USA).
  • EMBL - European Molecular Biology Laboratory (invited seminar, 2008, Heidelberg, Germany).
  • Bitplane Advanced Imaging Centers - Annual Meeting (invited partner, 2008, Zurich, Switzerland).
  • Institut Pasteur (invited seminar, 2008, Paris, France).
  • Institut Jacques Monod (invited seminar, 2008, Paris, France).
  • International Biophysics Congress (poster presentation, 2008, Long Beach, California, USA).
  • UCSB Workshop on Bio-Image Informatics: Biological Imaging, Computer Vision and Data Mining (poster presentation, 2008, Santa Barbara, California, USA).
  • Exciting Biologies 2007: Biology in Motion - Annual Meeting oganized by MGH/IPSEN/Cell Press - (oral presentation, 2007, Evian, France).
  • Bitplane Advanced Imaging Centers - Annual Meeting (invited partner, 2007, San Juan, Puerto Rico).
  • IUSTI - Ecole Polytechnique Universitaire de Marseille -Departement de Mécanique (invited seminar, 2006, Marseille, France).
  • UC Berkeley - Department of Cell and Molecular Biology - Isacoff Lab (interview, 2006, Berkeley, California, USA).
  • SPIE International Symposium - Photonics West (oral presentation, 2006, San Jose, California, USA).
  • Lawrence Livermore National Lab - Physical Biosciences Institute (invited seminar, 2005, Livermore, California, USA).
  • UC Berkeley - Bioengineering Department - Kumar Lab (interview, 2005, Berkeley, California, USA).
  • California Institute of Technology - Biological Imaging Center - Fraser Lab (interview, 2005, Pasadena, California, USA).
  • Orsay University - Soft Matter Department (invited seminar, 2005, Orsay, France).
  • Paris University - Matter and Complex Systems Lab (invited seminar, 2005, Paris, France).
  • CNRS School for Interdisciplinary Training Courses in Biology (invited speaker, 2005, Ile de Berder, France).
  • FOM: Focus On Microscopy (oral presentation, 2005, Jena, Germany).
  • IMVIE2 International Symposium - Imaging for Medical and Life Sciences (oral presentation, 2005, Illkirch/Strasbourg, France).
  • Seminaires des doctorants en chimie de l'Ecole Normale Supérieure (invited seminar, 2005, Paris, France).
  • 2emes Journees Scientifiques et Medicales de l'Institut Curie (oral presentation, 2005, Paris, France).
  • Institut Jacques Monod (invited seminar, 2004, Paris, France).
  • SPIE International Syposium - Photonics Euope (oral presentation, 2004, Paris, France).
  • French Society of Biomechanics, 29th Annual Meeting (oral presentation, 2004, Créteil, France).
  • Les Houches Summerschool on Optics and Biology (oral presentation, 2003, Les Houches, France).
  •  

     

     

    Conference Proceedings & Abstracts

     
    Supatto W, Fraser SE, Vermot J: Probing cilia-driven flow in living embryos using femtosecond laser ablation and fast imaging, Proc. SPIE - Laser Application in Micro-organisms and Biotechnology, 7203, 72030B (2009). Google Scholar | SPIE DL  
     
    Supatto W, McMahon A, Fraser SE, Stathopoulos A:
    Quantitative imaging of the collective cell movements shaping an embryo, Record of the 42nd Asilomar IEEE Conference on Signals, Systems and Computers - Advances in Bioimaging and Analysis, 61-63 (2009). IEEE Xplore
     
    Stathopoulos A, McMahon A, Supatto W, and Fraser SE: A quantitative analysis of imaging data provides insights into the coordination of cell movements during Drosophila gastrulation, Developmental Biology, 319(2): 476 (2008). ScienceDirect
     
    Supatto W, Brouzes E, Perrimon N, Fraser SE: Statistical analysis of cell movements during Drosophila embryo morphogenesis, Biophysical Journal, 94: 3194 (2008). Biophysical Journal website
     
    Supatto W, Debarre D, Moulia B, Farge E, and Beaurepaire E: An all-optical approach to modulate and quantitatively analyse embryo morphogenetic movements by using ultrashort laser pulses, Proc. SPIE - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues, 6088, 60881D (2006). Google Scholar | SPIE DL
     
    Debarre D, Supatto W, and Beaurepaire E: Structure sensitivity and sources of contrast in third-harmonic generation (THG) microscopy of cells and tissues, Proc. SPIE - Multiphoton Microscopy in the Biomedical Sciences, 6089, 60891H (2006). Google Scholar | SPIE DL
     
    Supatto W, Debarre D, Moulia B, Brouzes E, Martin JL, Schanne-Klein MC, Farge E, and Beaurepaire E: in vivo analysis of Drosophila embryo developmental dynamics by femtosecond pulse-induced ablation and multimodal nonlinear microscopy, Proc. SPIE - Multiphoton Microscopy in the Biomedical Sciences V, 5700, 256-264 (2005). Google Scholar | SPIE DL
     
    Supatto W, Brouzes E, Farge E, and Beaurepaire E: in vivo micro-dissection and live embryo imaging using two-photon microscopy to study Drosophila melanogaster early developement, Proc. SPIE - Femtosecond Laser Applications in Biology, 5463, 13-20 (2004). Google Scholar | SPIE DL
     
    Brouzes E, Supatto W, Beaurepaire E, Farge E: Mechanical induction of twist at early Drosophila embryo gastrulation, 29ème Congrès de la Société de Biomécanique, Arch. Physiol. Biochem., 112 Supplement, 4 (2004).
     
    Supatto W, Brouzes E, Beaurepaire E, Moulia B, Farge E: Kinematic analysis of morphogenetic movements involved in mechanically induced gene expression during Drosophila early development, 29ème Congrès de la Société de Biomécanique, Arch. Physiol. Biochem., 112 Supplement, 77 (2004).